[Expression profile of heat shock proteins in tissues and cells of lung adenocarcinoma].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 32(4): 660-4, 2007 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-17767061
ABSTRACT
OBJECTIVE:
To observe the expression profile of heat shock proteins (HSPs) including HSP70, inducible HSP90 (HSP86) and aB-crystallin in cells and tissues of lung adenocarcinoma.METHODS:
Western blotting and reverse transcriptional-polymerase chain reaction (RT-PCR) were performed to detect the expression of HSP70, HSP86 and aB-crystallin both in the protein and mRNA level respectively.RESULTS:
Compared with normal lung tissue and human bronchial epithelium (HBE) cells, RT-PCR and Western blotting showed that the expression of HSP70, HSP86 and alphaB crystallin increased significantly in both the mRNA and protein level in the cancer tissue and A549 human lung adenocarcinoma cells. Among the 3 sub-families of HSPs, the expression of HSP70 mRNA and protein increased most in both the lung tissue of cancer and A549 human adenocarcinoma cell lines.CONCLUSION:
The expression of HSPs is higher in the lung adenocarcinoma and A549 cells than that in the normal lung tissues and HBE cells. Among the HSP family, HSP70 is the most up-regulated member in the tissue and cells of lung adenocarcinoma.
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
/
Proteínas de Choque Térmico
/
Pulmão
/
Neoplasias Pulmonares
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article