p40phox as an alternative organizer to p47phox in Nox2 activation: a new mechanism involving an interaction with p22phox.
FEBS Lett
; 581(23): 4533-8, 2007 Sep 18.
Article
em En
| MEDLINE
| ID: mdl-17803994
ABSTRACT
p40(phox) activated phagocyte NADPH oxidase without p47(phox) in a cell-free system consisting of p67(phox), Rac and cytochrome b(558) relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47(phox). Addition of p47(phox) slightly increased the activation, but not additively. p40(phox) improved the efficiency of p67(phox) in the activation. The C-terminus-truncated p67(phox), p40(phox)(D289A), p40(phox)(R58A), or p40(phox)(W207R) showed an impaired activation. A peptide corresponding to the p22(phox) Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40(phox) SH3 domain. Thus, p40(phox) can substitute for p47(phox) in the activation, interacting with p22(phox) and p67(phox) through their specific regions.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
NADPH Oxidases
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article