Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.
Am J Obstet Gynecol
; 197(5): 516.e1-6, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17826730
ABSTRACT
OBJECTIVE:
Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is modulated by fatty acid-binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts. STUDYDESIGN:
Primary term human trophoblasts were cultured for 72 hours in either standard (O2 = 20%) or hypoxic (O2 < 1%) conditions. FABP expression was interrogated using polymerase chain reaction and Western immunoblotting. Trophoblast lipid droplets were examined using dipyrromethene boron difluoride 493/503 staining.RESULTS:
We detected the expression of FABP1, -3, -4, -5, and pm but not FABP2 or FABP6-9 subtypes in trophoblasts. Exposure to hypoxia markedly increased lipid droplet accumulation in trophoblasts. Consistent with this observation, hypoxia enhanced the expression of FABP1, -3, and -4. Lastly, agonists of peroxisome proliferator-activated receptor-gamma enhanced the expression of FABP1 and -4 in trophoblasts.CONCLUSION:
Hypoxia enhances the expression of FABP1, -3, and -4 in term human trophoblasts, suggesting that FABPs support fat accumulation in the hypoxic placenta.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
/
Proteínas de Ligação a Ácido Graxo
/
Hipóxia
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article