Katanin regulates dynamics of microtubules and biogenesis of motile cilia.
J Cell Biol
; 178(6): 1065-79, 2007 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-17846175
ABSTRACT
The in vivo significance of microtubule severing and the mechanisms governing its spatial regulation are not well understood. In Tetrahymena, a cell type with elaborate microtubule arrays, we engineered null mutations in subunits of the microtubule-severing complex, katanin. We show that katanin activity is essential. The net effect of katanin on the polymer mass depends on the microtubule type and location. Although katanin reduces the polymer mass and destabilizes the internal network of microtubules, its activity increases the mass of ciliary microtubules. We also show that katanin reduces the levels of several types of post-translational modifications on tubulin of internal and cortical microtubules. Furthermore, katanin deficiencies phenocopy a mutation of beta-tubulin that prevents deposition of polymodifications (glutamylation and glycylation) on microtubules. We propose that katanin preferentially severs older, post-translationally modified segments of microtubules.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Protozoários
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Tetrahymena thermophila
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Adenosina Trifosfatases
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Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article