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The differential effects of superoxide anion, hydrogen peroxide and hydroxyl radical on cardiac mitochondrial oxidative phosphorylation.
Zini, Roland; Berdeaux, Alain; Morin, Didier.
Afiliação
  • Zini R; INSERM, U841, Créteil, F-94010, France.
Free Radic Res ; 41(10): 1159-66, 2007 Oct.
Article em En | MEDLINE | ID: mdl-17886038
The involvement of reactive oxygen species (ROS) in cardiac ischemia-reperfusion injuries is well-established, but the deleterious effects of hydrogen peroxide (H(2)O(2)), hydroxyl radical (HO*) or superoxide anion (O(2)*(-) ) on mitochondrial function are poorly understood. Here, we report that incubation of rat heart mitochondria with each of these three species resulted in a decline of the ADP-stimulated respiratory rate but not substrate-dependent respiration. These three species reduced oxygen consumption induced by an uncoupler without alteration of the respiratory chain complexes, but did not modify mitochondrial membrane permeability. HO* slightly decreased F1F0-ATPase activity and HO* and O(2)*(-) partially inhibited the activity of adenine nucleotide translocase; H(2)O(2) failed to alter these targets. They inhibited NADH production by acting specifically on aconitase for O(2)*(-) and alpha-ketoglutarate dehydrogenase for H(2)O(2) and HO*. Our results show that O(2)*(-), H(2)O(2) and HO* act on different mitochondrial targets to alter ATP synthesis, mostly through inhibition of NADH production.
Assuntos
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Base de dados: MEDLINE Assunto principal: Fosforilação Oxidativa / Superóxidos / Ânions / Mitocôndrias / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Fosforilação Oxidativa / Superóxidos / Ânions / Mitocôndrias / Miocárdio Limite: Animals Idioma: En Ano de publicação: 2007 Tipo de documento: Article