Putative markers of infective life stages in Leishmania (Viannia) braziliensis.
Parasitology
; 134(Pt 12): 1689-98, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17897481
ABSTRACT
Gene expression is known to vary significantly during the Leishmania life-cycle. Its monitoring might allow identification of molecular changes associated with the infective stages (metacyclics and amastigotes) and contribute to the understanding of the complex host-parasite relationships. So far, very few studies have been done on Leishmania (Viannia) braziliensis, one of the most pathogenic species. Such studies require, first of all, reference molecular markers. In the present work, we applied differential display analysis (DD analysis) in order to identify transcripts that might be (i) candidate markers of metacyclics and intracellular amastigotes of L. (V.) braziliensis or (ii) potential controls, i.e. constitutively expressed. In total, 48 DNA fragments gave reliable sequencing data, 29 of them being potential markers of infective stages and 12 potential controls. Eight sequences could be identified with reported genes. Validation of the results of DD analysis was done for 4 genes (2 differentially expressed and 2 controls) by quantitative real-time PCR. The infective insect stage-specific protein (meta 1) was more expressed in metacyclic-enriched preparations. The oligopeptidase b showed a higher expression in amastigotes. Two genes, glucose-6-phosphate dehydrogenase and a serine/threonine protein kinase, were found to be similarly expressed in the different biological samples.
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Base de dados:
MEDLINE
Assunto principal:
Leishmania braziliensis
/
Regulação da Expressão Gênica
/
Leishmaniose Cutânea
/
Genes de Protozoários
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article