Evaluation of two Toxoplasma gondii serologic tests used in a serosurvey of domestic cats in California.
J Parasitol
; 93(4): 806-16, 2007 Aug.
Article
em En
| MEDLINE
| ID: mdl-17918359
ABSTRACT
We evaluated the sensitivity (Se) and specificity (Sp) of an IgG enzyme-linked immunosorbent assay (ELISA) and IgG indirect fluorescent antibody test (IFAT) for detection of Toxoplasma gondii-specific antibodies in sera from 2 cat populations using a Bayesian approach. Accounting for test covariance, the Se and Sp of the IgG ELISA were estimated to be 92.6% and 96.5%, and those of the IgG IFAT were 81.0% and 93.8%, respectively. Both tests performed poorly in cats experimentally coinfected with feline immunodeficiency virus and T. gondii. Excluding this group, Se and Sp of the ELISA were virtually unchanged (92.3% and 96.4%, respectively), whereas the IFAT Se improved to 94.2% and Sp remained stable at 93.7%. These tests and an IgM ELISA were applied to 123 cat sera from the Morro Bay area, California, where high morbidity and mortality attributable to toxoplasmosis have been detected in southern sea otters. Age-adjusted IgG seroprevalence in this population was estimated to be 29.6%, and it did not differ between owned and unowned cats. Accounting for Se, Sp, and test covariances, age-adjusted seroprevalence was 45.0%. The odds for T. gondii seropositivity were 12.3-fold higher for cats aged >12 mo compared with cats aged <6 mo.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Antiprotozoários
/
Doenças do Gato
/
Toxoplasmose Animal
/
Técnica Indireta de Fluorescência para Anticorpo
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Evaluation_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
País como assunto:
America do norte
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article