Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.
Cell
; 131(2): 271-85, 2007 Oct 19.
Article
em En
| MEDLINE
| ID: mdl-17956729
ABSTRACT
The chromosomal passenger complex (CPC) is a key regulator of chromosome segregation and cytokinesis. CPC functions are connected to its localization. The complex first localizes to centromeres and later associates with the central spindle and midbody. Survivin, Borealin, and INCENP are the three components of the CPC that regulate the activity and localization of its enzymatic component, the kinase Aurora B. We determined the 1.4 A resolution crystal structure of the regulatory core of the CPC, revealing that Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle. We used siRNA rescue experiments with structure-based mutants to explore the requirements for CPC localization. We show that the intertwined structural interactions of the core components lead to functional interdependence. Association of the core "passenger" proteins creates a single structural unit, whose composite molecular surface presents conserved residues essential for central spindle and midbody localization.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Cromossômicas não Histona
/
Proteínas de Ciclo Celular
/
Segregação de Cromossomos
/
Proteínas Associadas aos Microtúbulos
/
Fuso Acromático
/
Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article