Characterization of protein conjugates using capillary electrophoresis.
J Chromatogr A
; 1173(1-2): 159-64, 2007 Nov 30.
Article
em En
| MEDLINE
| ID: mdl-17964582
ABSTRACT
With the aim of generating antibodies, a calix[4]arene-crown-6 was coupled to bovine serum albumin. For that purpose, a complete procedure to optimize and characterize the coupling of hydrophobic haptens based on capillary electrophoresis (CE) was developed. We demonstrated the existence of a polynomial relationship between the electrophoretic mobility (mu(ep)) and the hapten density. This correlation was used not only to study the coupling reaction in terms of optimization and kinetics but also to determine the average coupling molar ratio of any given conjugate. An estimation of the heterogeneity of these conjugates by simulation of experimental peaks was also proposed.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Eletroforese Capilar
Limite:
Animals
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article