[Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549].
Bing Du Xue Bao
; 23(5): 360-5, 2007 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-17969852
ABSTRACT
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas não Estruturais Virais
/
Apoptose
/
Virus da Influenza A Subtipo H5N1
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article