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CD43 signals induce Type One lineage commitment of human CD4+ T cells.
Ramírez-Pliego, Oscar; Escobar-Zárate, Diana L; Rivera-Martínez, Gemma M; Cervantes-Badillo, Mayte G; Esquivel-Guadarrama, Fernando R; Rosas-Salgado, Gabriela; Rosenstein, Yvonne; Santana, M Angélica.
Afiliação
  • Ramírez-Pliego O; Facultad de Ciencias, Universidad Autónoma del Estado de Morelos, Av, Universidad 1001, Col, Chamilpa, Cuernavaca, 62210, Mexico. raplos2002@yahoo.com.mx
BMC Immunol ; 8: 30, 2007 Nov 23.
Article em En | MEDLINE | ID: mdl-18036228
BACKGROUND: The activation and effector phenotype of T cells depend on the strength of the interaction of the TcR with its cognate antigen and additional signals provided by cytokines and by co-receptors. Lymphocytes sense both the presence of an antigen and also clues from antigen-presenting cells, which dictate the requisite response. CD43 is one of the most abundant molecules on the surface of T cells; it mediates its own signalling events and cooperates with those mediated by the T cell receptor in T cell priming. We have examined the role of CD43 signals on the effector phenotype of adult CD4+ and CD8+ human T cells, both alone and in the presence of signals from the TcR. RESULTS: CD43 signals direct the expression of IFNgamma in human T cells. In freshly isolated CD4+ T cells, CD43 signals potentiated expression of the IFNgamma gene induced by TcR activation; this was not seen in CD8+ T cells. In effector cells, CD43 signals alone induced the expression of the IFNgamma gene in CD4+ T cells and to a lesser extent in CD8+ cells. The combined signals from CD43 and the TcR increased the transcription of the T-bet gene in CD4+ T cells and inhibited the transcription of the GATA-3 gene in both populations of T cells, thus predisposing CD4+ T cells to commitment to the T1 lineage. In support of this, CD43 signals induced a transient membrane expression of the high-affinity chains of the receptors for IL-12 and IFNgamma in CD4+ T cells. CD43 and TcR signals also cooperated with those of IL-12 in the induction of IFNgamma expression. Moreover, CD43 signals induced the co-clustering of IFNgammaR and the TcR and cooperated with TcR and IL-12 signals, triggering a co-capping of both receptors in CD4+ populations, a phenomenon that has been associated with a T1 commitment. CONCLUSION: Our results suggest a key role for CD43 signals in the differentiation of human CD4+ T cells into a T1 pattern.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Th1 / Linhagem da Célula / Leucossialina / Receptores de Interleucina-12 Limite: Adult / Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Th1 / Linhagem da Célula / Leucossialina / Receptores de Interleucina-12 Limite: Adult / Humans Idioma: En Ano de publicação: 2007 Tipo de documento: Article