Hepatitis B vaccine-specific CD4(+) T cells can be detected and characterised at the single cell level: limited usefulness of dendritic cells as signal enhancers.
J Immunol Methods
; 330(1-2): 1-11, 2008 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-18061610
ABSTRACT
Hepatitis B surface antigen (HBsAg)-specific T cells were analysed in 16 healthy individuals vaccinated against hepatitis B, using optimised protocols. HBsAg-specific IFN-gamma producing CD4(+) T cells were found at a similar low frequency (0.01%) within the naive T cell subset, the central and the effector memory T cell subset. Overall, HBsAg-specific total CD4(+) T cells were detected in approximately 81% of the HBV vaccinees. The use of dendritic cells substantially increased the otherwise low proliferative responses but not the percentage of IFN-gamma producing cells. The analysis of readily detectable CMV-specific CD4(+) T cell responses was used as a positive control and confirmed the adequacy of our assays. T cell responses associated with (in-) adequate hepatitis B vaccination can now be analysed in more detail.
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Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Ativação Linfocitária
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Linfócitos T CD4-Positivos
/
Subpopulações de Linfócitos T
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Vacinação
/
Vacinas contra Hepatite B
/
Anticorpos Anti-Hepatite B
/
Antígenos de Superfície da Hepatite B
Tipo de estudo:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article