Human cytomegalovirus uses two distinct pathways to enter retinal pigmented epithelial cells.
Proc Natl Acad Sci U S A
; 104(50): 20037-42, 2007 Dec 11.
Article
em En
| MEDLINE
| ID: mdl-18077432
ABSTRACT
Human cytomegalovirus infects multiple cell types, including fibroblasts and epithelial cells. It penetrates fibroblasts by fusion at the cell surface but is endocytosed into epithelial cells. In this report, we demonstrate by electron microscopy that the virus uses two different routes to enter retinal pigmented epithelial cells, depending on the cell type in which the infecting virus was produced. Virus produced in epithelial cells preferentially fuses with the plasma membrane, whereas fibroblast-derived virus mostly enters by receptor-mediated endocytosis. Treatment of epithelial cells with agents that block endosome acidification inhibited infection by virus produced in fibroblasts but had only a modest effect on infection by virus from epithelial cells. Epithelial cell-generated virions had higher intrinsic "fusion-from-without" activity than fibroblast-generated particles, and the two virus preparations triggered different cellular signaling responses, as evidenced by markedly different transcriptional profiles. We propose that the cell type in which a human cytomegalovirus particle is produced likely influences its subsequent spread and its contribution to pathogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado Ocular
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Retina
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Citomegalovirus
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Endocitose
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Células Epiteliais
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Internalização do Vírus
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article