Expression, purification and crystallization of human 5-lipoxygenase-activating protein with leukotriene-biosynthesis inhibitors.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 63(Pt 12): 1054-7, 2007 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-18084092
ABSTRACT
The nuclear membrane protein 5-lipoxygenase-activating protein (FLAP) plays an essential role in leukotriene synthesis. Recombinant full-length human FLAP with a C-terminal hexahistidine tag has been expressed and purified from the cytoplasmic membrane of Escherichia coli. Diffraction-quality crystals of FLAP in complex with leukotriene-synthesis inhibitor MK-591 and with an iodinated analogue of MK-591 have been grown using the sitting-drop vapor-diffusion method. The crystals exhibit tetragonal symmetry (P42(1)2) and diffracted to a resolution limit of 4 A.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Expressão Gênica
/
Leucotrienos
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article