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Ontogenesis of brain aromatase P450 expression in the bovine hypothalamus.
Peruffo, Antonella; Cozzi, Bruno; Ballarin, Cristina.
Afiliação
  • Peruffo A; Department of Experimental Veterinary Science, University of Padova, viale dell'Università 16, 35020 Legnaro (PD), Agripolis, Italy.
Brain Res Bull ; 75(1): 60-5, 2008 Jan 31.
Article em En | MEDLINE | ID: mdl-18158096
Aromatase P450 (P450(AROM)), converting testosterone (T) into estradiol (E), plays an important role in sexual differentiation of neural structures in the developing mammalian brain. The aim of the present study was to characterize the qualitative and quantitative profile of P450(AROM) mRNA expression in the bovine hypothalamus (the region of the central nervous system in which the enzyme is mainly localized) using RT-PCR and quantitative real-time RT-PCR analysis, respectively. P450(AROM) expression was examined in the developing hypothalamus in a series of experimental groups investigated at 10 weeks interval one from the other. Our data indicate that in the bovine fetal hypothalamus P450(AROM) expression peaks at the second quarter of gestation. The presence of neural cells containing P450(AROM) in the bovine fetal hypothalamus was confirmed by immunohistochemistry, and localized in the medial preoptic area. We conclude that second quarter of the gestation is the developmental stage which represents a critical period for hypothalamic differentiation in bovine ontogenesis, an important difference with the rat and mouse, short gestation species in which P450(AROM) activity peaks around delivery.
Assuntos
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Base de dados: MEDLINE Assunto principal: Aromatase / Regulação Enzimológica da Expressão Gênica / Hipotálamo Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Aromatase / Regulação Enzimológica da Expressão Gênica / Hipotálamo Tipo de estudo: Qualitative_research Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article