Influence of systematically varied nano-scale topography on cell morphology and adhesion.
Cell Commun Adhes
; 14(5): 181-94, 2007.
Article
em En
| MEDLINE
| ID: mdl-18163229
ABSTRACT
The types of cell-matrix adhesions and the signals they transduce strongly affect the cell-phenotype. We hypothesized that cells sense and respond to the three-dimensionality of their environment, which could be modulated by nano-structures on silicon surfaces. Human foreskin fibroblasts were cultured on nano-structures with different patterns (nano-post and nano-grate) and heights for 3 days. The presence of integrin alpha(5), beta(1), beta(3), paxillin and phosphorylated FAK (pFAK) were detected by western blot and immunofluorescence. Integrin beta(3) exhibited stronger signals on nano-grates. pFAK and paxillin were observed as small dot-like patterns on the cell-periphery on nano-posts and as elongated and aligned patterns on nano-grates. Collectively, our observations highlighted the presence of focal (integrin beta(1), beta(3), pFAK, paxillin), fibrillar (integrin alpha(5), beta(1)) and 3-D matrix (integrin alpha(5), beta(1), paxillin) adhesions on nano-structures. The presented nano-structures offer interesting opportunities to study the interaction of cells with topographical features comparable to the size of extracellular matrix components.
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Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Membrana Celular
/
Receptores de Superfície Celular
/
Nanoestruturas
/
Matriz Extracelular
Limite:
Humans
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article