Isolation and characterization of egg yolk antibodies IgY from hens immunized with different influenza virus strains.
Roum Arch Microbiol Immunol
; 50(3): 215-22, 1991.
Article
em En
| MEDLINE
| ID: mdl-1821163
ABSTRACT
A comparison of two precipitation methods of IgY from normal hen eggs was made. For method I the precipitation agent is represented by PEG 6000, and for method II by organic solvents. The comparative study of some parameters (protein concentration, ovalbumin content, presence of non-specific inhibitors, PAA-gel electrophoresis) shows that method I is more efficient and more convenient than method II. Using this method, we isolated and characterized IgY preparations from hens immunized with circulating influenza virus strains A/Singapore/6/86 (H1N1), A/Shanghai/11/87 (H3N2) B/Beijing/1/87 and B/Yamagata/16/88 These viral IgY antibody preparations are homogeneous, lacking anti-host cell antibodies and non-specific inhibitors. Their NI titres and HI titres are higher than those found in the sera of immunized birds.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Vírus da Influenza B
/
Imunoglobulinas
/
Galinhas
/
Imunização
/
Gema de Ovo
/
Anticorpos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article