Assembly and micromanipulation of Xenopus in vitro-assembled mitotic chromosomes.
Curr Protoc Cell Biol
; Chapter 22: Unit 22.9, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-18228470
ABSTRACT
Mitotic chromosomes have fascinated scientists for several decades. Despite the numerous microscopy studies, chromosome structure is, however, still poorly understood. This is due to both the high complexity of the mitotic chromosomes and the lack of other appropriate techniques suitable for studying their organization. This unit describes a novel physical approach based on measurements of mitotic chromosome elasticity. The elasticity properties are determined by the underlying structure, and knowledge of them has allowed a description of the organization of the mitotic chromosomes and critical analysis of the available models. In this unit, a detailed protocol for the measurements of the elastic response of in vitro assembled mitotic chromosomes in Xenopus egg extract is presented.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromossomos
/
Micromanipulação
/
Microscopia
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article