A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays.
Rev Sci Instrum
; 79(1): 013708, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-18248040
ABSTRACT
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
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Base de dados:
MEDLINE
Assunto principal:
Robótica
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Aumento da Imagem
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Microscopia Confocal
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Análise em Microsséries
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article