Short communication: characterization of a kappa-casein genetic variant in the Chinese yak, Bos grunniens.
J Dairy Sci
; 91(3): 1204-8, 2008 Mar.
Article
em En
| MEDLINE
| ID: mdl-18292277
ABSTRACT
A PCR-single strand conformation polymorphism protocol has been developed for rapid genotyping of the yak kappa-casein gene. A total of 307 yaks from the Tianzhu White, Jiulong, Maiwa, and Datong breeds in China were genotyped at the kappa-casein locus using the protocol developed in the present study. A polymorphism of kappa-casein gene exon 4 has been identified in Tianzhu White breed by evaluating genomic DNA. The polymorphic site consists of a single nucleotide substitution G-->C at position 362 of the exon 4, resulting in an AA substitution from Arg to Pro at position 121 of the AA sequence and in 2 alleles named, respectively, G and C based on nucleotide 362. The occurrence of allele C in the Tianzhu White breed was high with an allele frequency of 0.15. However, allele C appears to be absent in the yaks from Jiulong, Maiwa, and Datong breeds.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Bovinos
/
Caseínas
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article