Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken.
Vet Immunol Immunopathol
; 122(3-4): 275-84, 2008 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-18304651
ABSTRACT
The objective of the study was to determine the cellular and cytokine responses associated with dinitrofluorobenzene (DNFB)-induced skin contact hypersensitivity (SCH), as an indicator of cell-mediated immune response, in the chicken. The thickness of the DNFB-treated foot web was increased by 6h.p.i. (hours post-induction), peaked by 24h.p.i. and then declined gradually until the lowest measurements were observed at 72h.p.i. Infiltration of eosinophils was the highest at 6 and 12h.p.i. and gradually declined by 48h.p.i. The degree of infiltration of both CD8+ and CD4+ T cells varied with mild infiltration observed at 6h.p.i., moderate to heavy infiltration observed at 12h.p.i. that persisted through 24 and 48h.p.i. and declined by 72h.p.i. Infiltration of macrophages during the study period was prominent, yet less remarkable differences were recorded between observations. Expression of interleukin (IL)-4, IL-6, IL-10 and interferon (IFN)-gamma in skin tissue was at its highest at 6h.p.i. compared to other observed time points, yet only the expression of IFN-gamma and IL-10 genes turned out to be significantly higher at 6h.p.i. compared to all other time points. In conclusion, DNFB-induced SCH in chicken was associated with an early up-regulation of cytokine genes, and infiltration of eosinophils along with macrophages, CD8+, and CD4+ T cells at the site of induction.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Galinhas
/
Citocinas
/
Dermatite de Contato
/
Dinitrofluorbenzeno
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article