IMPY, a potential beta-amyloid imaging probe for detection of prion deposits in scrapie-infected mice.
Nucl Med Biol
; 35(2): 197-201, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18312829
ABSTRACT
INTRODUCTION:
A potential single-photon emission computed tomography imaging agent for labeling of A beta plaques of Alzheimer's disease, IMPY (2-(4'-dimethylaminophenyl)-6-iodo-imidazo[1,2-a]pyridine), would be effective in detection of prion amyloid deposits in transmissible spongiform encephalopathies (TSEs).METHODS:
In vitro autoradiographic studies were carried out with [125 I]IMPY on brain sections from scrapie-infected mice and age-matched controls. Competition study was performed to evaluate the prion deposit binding specificity with nonradioactive IMPY.RESULTS:
Binding of [125 I]IMPY was observed in infected brain sections, while on age-matched control brain sections, there was no or very low labeling. Prion deposit binding was confirmed by histoblots with prion protein-specific monoclonal antibody 2D6. In the presence of nonradioactive IMPY, the binding of [125 I]IMPY was significantly inhibited in all regions studied.CONCLUSIONS:
These findings indicate that IMPY can detect the prion deposits in vitro in scrapie-infected mice. Labeled with 123 I, this ligand may be useful to quantitate prion deposit burdens in TSEs by in vivo imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirazóis
/
Scrapie
/
Príons
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Peptídeos beta-Amiloides
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Placa Amiloide
/
Marcação por Isótopo
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article