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Refinement of strategies for the development of a human cytomegalovirus dense body vaccine.
Mersseman, Véronique; Böhm, Verena; Holtappels, Rafaela; Deegen, Petra; Wolfrum, Uwe; Plachter, Bodo; Reyda, Sabine.
Afiliação
  • Mersseman V; Institute for Virology, Johannes Gutenberg-Universität, Obere Zahlbacher Str. 67, 55101 Mainz, Germany.
Med Microbiol Immunol ; 197(2): 97-107, 2008 Jun.
Article em En | MEDLINE | ID: mdl-18320219
Development of a vaccine against human cytomegalovirus (HCMV) infection has been identified as a high priority goal in biomedical research, yet no vaccine has been licensed until now. Recombinant subviral dense bodies (recDB) are a promising basis for the establishment of such a vaccine. In this article, strategies for the generation of recDB, based on recombination-mediated genetic engineering of the 230 kb HCMV DNA genome in E. coli are outlined. Analysis of viral mutants that were constructed in this process provided the proof-of-principle that heterologous antigens can be packaged into recDB and that these particles prime CD8 T cell responses against the recombinant antigen upon their application to HLA-A2 transgenic mice.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Vacinas contra Citomegalovirus / Citomegalovirus Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Vacinas contra Citomegalovirus / Citomegalovirus Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article