[Detection the coinfection of Hantavirus and Orientia tsutsugamushi in primary cultured mite cells].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 21(4): 307-9, 2007 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-18322585
ABSTRACT
OBJECTIVE:
To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).METHODS:
Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.RESULTS:
Positive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.CONCLUSION:
Coinfection of HV and Ot did exist in wild Leptotrombidium scutellare.
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Base de dados:
MEDLINE
Assunto principal:
Orientia tsutsugamushi
/
Orthohantavírus
/
Ácaros
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2007
Tipo de documento:
Article