Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts.
Free Radic Biol Med
; 44(10): 1817-32, 2008 May 15.
Article
em En
| MEDLINE
| ID: mdl-18329388
ABSTRACT
Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Senescência Celular
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Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina
/
Diploide
/
Fibroblastos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article