Identification of conjugated estrogen metabolites in dog plasma following administration of estriol-2,4,6,7-3H.

ABSTRACT

PIP: Conjugated estrogen metabolites in arterial plasma were identified in male dogs by solvent partition, DEAE-Sephadex, Celite partition, and thin layer chromatography following constant infusion of tritiated-2,4,6,7-estriol for 90 minutes. Identification was confirmed by specific activity assessment in various solvent systems, enzyme hydrolysis and chromatography of the unconjugated steroids and their derivatives. All unconjugated and radioactivity after infusion was identified as free estriol, and 5 conjugated metabolites were isolated. 3 of these were identified as estriol-3-glucosiduronate, estriol-16alpha-glucosiduronate, and estriol-3-sulfate. 2 polyconjugates of estriol appeared to be estriol-3-sulfate-16alpha glucosiduronate and an estriol diglucosiduronate.