The role of the Saccharomyces cerevisiae Cdc7-Dbf4 complex in the replication checkpoint.
Gene
; 414(1-2): 32-40, 2008 May 15.
Article
em En
| MEDLINE
| ID: mdl-18372119
ABSTRACT
The Cdc7-Dbf4 complex is a conserved serine/threonine protein kinase essential for the initiation of eukaryotic DNA replication. Although an mcm5-bob1 mutation bypasses lethality conferred by mutations in CDC7 or DBF4, the Deltacdc7 mcm5-bob1 mutant is sensitive to hydroxyurea (HU), which induces replication stress. To elucidate the reasons for HU sensitivity conferred by deletion of CDC7, we examined the role of Cdc7-Dbf4 in the replication checkpoint. We found that in Cdc7-Dbf4-deficient cells exposed to replication stress, Rad53 remains in a hypophosphorylated form, anaphase spindle is elongated, and checkpoint-specific transcription is not induced. The hypophosphorylated Rad53 exhibits a low autophosphorylation activity, and recombinant Cdc7-Dbf4 phosphorylates Rad53 in vitro. These results suggest that Cdc7-Dbf4 is required for full activation of Rad53 in response to replication stress.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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DNA Fúngico
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Proteínas Serina-Treonina Quinases
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Proteínas de Ciclo Celular
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Proteínas de Saccharomyces cerevisiae
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Replicação do DNA
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article