"On the Collander equation": protein partitioning in polymer/polymer aqueous two-phase systems.
J Chromatogr A
; 1190(1-2): 39-43, 2008 May 09.
Article
em En
| MEDLINE
| ID: mdl-18378246
ABSTRACT
Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation lnKi=aiolnKo+bio, where Ki and Ko are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients aio and bio are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Proteínas
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article