Effect of high throughput RHD typing of fetal DNA in maternal plasma on use of anti-RhD immunoglobulin in RhD negative pregnant women: prospective feasibility study.
BMJ
; 336(7648): 816-8, 2008 Apr 12.
Article
em En
| MEDLINE
| ID: mdl-18390496
ABSTRACT
OBJECTIVES:
To assess the feasibility of applying a high throughput method, with an automated robotic technique, for predicting fetal RhD phenotype from fetal DNA in the plasma of RhD negative pregnant women to avoid unnecessary treatment with anti-RhD immunoglobulin.DESIGN:
Prospective comparison of fetal RHD genotype determined from fetal DNA in maternal plasma with the serologically determined fetal RhD phenotype from cord blood.SETTING:
Antenatal clinics and antenatal testing laboratories in the Midlands and north of England and an international blood group reference laboratory.PARTICIPANTS:
Pregnant women of known gestation identified as RhD negative by an antenatal testing laboratory. Samples from 1997 women were taken at or before the 28 week antenatal visit. MAIN OUTCOMEMEASURES:
Detection rate of fetal RhD from maternal plasma, error rate, false positive rate, and the odds of being affected given a positive result.RESULTS:
Serologically determined RhD phenotypes were obtained from 1869 cord blood samples. In 95.7% (n=1788) the correct fetal RhD phenotype was predicted by the genotyping tests. In 3.4% (n=64) results were either unobtainable or inconclusive. A false positive result was obtained in 0.8% (14 samples), probably because of unexpressed or weakly expressed fetal RHD genes. In only three samples (0.2%) were false negative results obtained. If these results had been applied as a guide to treatment, only 2% of the women would have received anti-RhD unnecessarily, compared with 38% without the genotyping.CONCLUSIONS:
High throughput RHD genotyping of fetuses in all RhD negative women is feasible and would substantially reduce unnecessary administration of anti-RhD immunoglobulin to RhD negative pregnant women with an RhD negative fetus.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Isoimunização Rh
/
DNA
/
Imunoglobulina rho(D)
/
Doenças Fetais
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article