Biochemical alterations in juvenile carp (Cyprinus carpio) exposed to zinc: glutathione reductase as a target.
Mar Environ Res
; 66(1): 88-9, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18395787
ABSTRACT
The aim of this study was to investigate biochemical changes in juvenile carp (Cyprinus carpio) exposed to zinc chloride (10, 30 and 100 microM) for a period of 48 h. Zinc exposure caused a concentration-dependent reduction in glutathione reductase (GR) activity in gills, liver and brain. Gill glutathione S-transferase (GST) was reduced when animals were exposed to the highest concentration of 100 microM zinc. The phosphorylation of p38(MAPK) increased in the brain of fish exposed to zinc 100 microM, while phosphorylation of the extracellular signal-regulated protein kinase 1/2 (ERK1/2) and c-Jun N-terminal protein kinase 1/2 (JNK1/2) remained unchanged. Expression of proteins HSP60 and HSP70 were not affected by zinc exposure. Considering the significant concentration-dependent inhibition of GR in all tissues analyzed, this enzyme could be a potential biomarker of exposure to zinc, which has to be confirmed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Carpas
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Cloretos
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Compostos de Zinco
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Brânquias
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Glutationa Redutase
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Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article