Chemical reagents for investigating the major groove of DNA.
Curr Protoc Nucleic Acid Chem
; Chapter 6: Unit 6.6, 2001 Aug.
Article
em En
| MEDLINE
| ID: mdl-18428867
ABSTRACT
Chemical modification provides an inexpensive and rapid method for characterizing the structure of DNA and its association with drugs and proteins. Numerous conformation-specific probes are available, but most investigations rely on only the most common and readily available of these. The major groove of DNA is typically characterized by reaction with dimethyl sulfate, diethyl pyrocarbonate, potassium permanganate, osmium tetroxide, and, quite recently, bromide with monoperoxysulfate. This commentary discusses the specificity of these reagents and their applications in protection, interference, and missing contact experiments.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bioquímica
/
DNA
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article