Estimation of chromosomal imbalances in preimplantation embryos from preimplantation genetic diagnosis cycles of reciprocal translocations with or without acrocentric chromosomes.
Fertil Steril
; 90(6): 2144-51, 2008 Dec.
Article
em En
| MEDLINE
| ID: mdl-18440525
OBJECTIVE: To estimate chromosomal imbalances in preimplantation embryos from reciprocal translocation carriers with or without acrocentric chromosomes (Acro-Ch) 13, 14, 15, 21, and 22 in preimplantation genetic diagnosis (PGD) cycles. DESIGN: Fluorescence in situ hybridization was applied to PGD cycles for reciprocal translocation carriers. SETTING: University-based centers for reproductive medicine. PATIENT(S): Ten and 24 patients of reciprocal translocation with and without Acro-Ch, respectively. INTERVENTION(S): Fluorescence in situ hybridization in biopsied blastomeres. MAIN OUTCOME MEASURE(S): Estimation of meiotic segregation mode in embryos from translocation carriers. RESULT(S): The proportion of alternative segregation for normal or balanced chromosome contents in preimplantation embryos from PGD cycles in reciprocal translocations without Acro-Ch was significantly higher than that with Acro-Ch (26.0% vs. 14.6%). The proportion of interchange trisomy in 3:1 segregation was significantly lower in reciprocal translocations without Acro-Ch than that with Acro-Ch (4.3% vs. 9.5%). CONCLUSION(S): This is the first report that the incidence of alternative segregation producing normal or balanced embryos was relatively low in reciprocal translocations associated with Acro-Ch. Our data may be useful to predict the possibility of normal or balanced embryos and to counsel with reciprocal translocation carriers for PGD-fluorescence in situ hybridization cycles.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
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Testes Genéticos
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Cromossomos Humanos
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Hibridização in Situ Fluorescente
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Diagnóstico Pré-Implantação
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Segregação de Cromossomos
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Técnicas de Reprodução Assistida
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Infertilidade
Tipo de estudo:
Diagnostic_studies
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Etiology_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Pregnancy
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article