Parameters for DNA vaccination using adaptive constant-current electroporation in mouse and pig models.
Vaccine
; 26(40): 5230-7, 2008 Sep 19.
Article
em En
| MEDLINE
| ID: mdl-18450333
ABSTRACT
Enhancing the expression of DNA vaccines requires that specific conditions of delivery are optimized. We describe experiments using adaptive constant-current electroporation (EP) in mice and pigs examining parameters such as target muscle, delay between plasmid delivery and onset of EP pulses and DNA vaccine formulation; our studies show that concentrated formulations result in better expression and immunogenicity. Furthermore, various conditions of EP that limit the amount of muscle damage were measured. The results of these studies will help to advance the success of DNA vaccines in animals into success in human clinical trials.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Eletroporação
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Vacinas de DNA
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Proteínas de Fluorescência Verde
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Fosfatase Alcalina
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Anticorpos
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Músculos
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article