Detection of Trypanosoma brucei parasites in blood samples using real-time nucleic acid sequence-based amplification.
Diagn Microbiol Infect Dis
; 61(4): 440-5, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18486402
ABSTRACT
Currently, the conventional diagnosis of human African trypanosomiasis (HAT) is by microscopic demonstration of trypomastigotes in blood, lymph, and/or cerebrospinal fluid. However, microscopic diagnosis of HAT is not sensitive enough and may give false-negative results, thus, denying the patient the necessary treatment of the otherwise fatal disease. For this reason, a highly sensitive technique needs to be developed to enhance case findings. In this study, the real-time nucleic acid sequence-based amplification assay described is based on amplification and concurrent detection of small subunit rRNA (18S rRNA) of Trypanosoma brucei. The sensitivity of the assay was evaluated on nucleic acid from in vitro cultured parasites and blood spiked with various parasites quantities. The assay detected 10 parasites/mL using cultured parasites as well as spiked blood. A sensitive assay such as the one developed in this study may become an alternative tool to confirm diagnosis of human African trypanosomiasis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma brucei brucei
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Tripanossomíase Africana
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Sangue
/
Replicação de Sequência Autossustentável
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article