[An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization].
Zhonghua Nan Ke Xue
; 14(3): 211-4, 2008 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-18488331
ABSTRACT
OBJECTIVE:
To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.METHODS:
The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.RESULTS:
Of the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).CONCLUSION:
Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 18
/
Coloração Cromossômica
/
Cromossomos Humanos X
/
Cromossomos Humanos Y
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Astenozoospermia
Limite:
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article