Development and validation of an HPLC-UV method for iodixanol quantification in human plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
; 869(1-2): 133-7, 2008 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-18502708
ABSTRACT
Iodixanol is a widely used iso-osmolar contrast medium agent. Similar to iohexol, it can also be a good exogenous marker for the measurement of glomerular filtration rate (GFR). This article describes the development and validation of an HPLC-UV method for quantification of iodixanol in human plasma. Internal standard, iohexol (20 microl, 1 mg/ml), and perchloric acid (30 microl, 20%, v/v) were added to plasma samples (300 microl), followed by neutralization with 10 microl potassium carbonate (5M). Samples were centrifuged and 10 microl of the supernatant was injected onto a C(18) EPS analytical column (3 microm particle size, 150 mm x 4.6 mm). The extraction method yielded >95% recovery for both iodixanol and iohexol. The mobile phase consisted of 0.1% (w/v) sodium formate buffer and acetonitrile. Iohexol and iodixanol peaks were eluted at approximately 5 and 9 min, respectively using a fast gradient method. The assay lower limit of detection was 2.0 microg/ml and lower limit of quantification was 10 microg/ml. The calibration curves, assessed in six replicates, were linear over an iodixanol concentration range of 10-750 microg/ml. Intra- and inter-day accuracy was >95% and precision expressed as % coefficient of variation was <10%. This method is simple, accurate, precise and robust and can potentially be used for iodixanol quantification in large-scale clinical studies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Tri-Iodobenzoicos
/
Cromatografia Líquida de Alta Pressão
/
Meios de Contraste
Limite:
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article