[Simultaneous determination of ochratoxin A, B and citrinin in foods by HPLC-FL and LC/MS/MS].
Shokuhin Eiseigaku Zasshi
; 49(2): 100-5, 2008 Apr.
Article
em Ja
| MEDLINE
| ID: mdl-18503246
ABSTRACT
Methods using high-performance liquid chromatography with fluorescence detection (HPLC-FL) and using liquid chromatography with tandem mass spectrometry (LC/MS/MS) were developed for simultaneous determination of ochratoxin A (OTA), ochratoxin B (OTB) and citrinin (CIT) in cereal, fruit, and coffee products. The samples were extracted with ethyl acetate under an acidic condition, and then cleaned up with liquid-liquid separation. The test solutions were analyzed by reverse-phase HPLC-FL and LC/MS/MS. Mass spectral acquisition was performed in positive ion mode by applying multiple reaction monitoring. The performances of both detectors were almost equivalent. The recoveries of OTA and OTB were 87-111%, and that of CIT were 70-88%. The limits of quantification (S/N> or =10) of OTA, OTB and CIT was 0.1 mug/kg or less. These methods were considered to be useful for the determination of the three mycotoxins at low levels (0.1 microg/kg).
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Contaminação de Alimentos
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia Líquida
/
Citrinina
/
Espectrometria de Massas em Tandem
/
Análise de Alimentos
/
Ocratoxinas
Idioma:
Ja
Ano de publicação:
2008
Tipo de documento:
Article