Ionic liquids and proteases: a clean alliance for semisynthesis.
Chembiochem
; 9(9): 1493-9, 2008 Jun 16.
Article
em En
| MEDLINE
| ID: mdl-18509837
ABSTRACT
Herein we present the first report on protease-catalysed ligation of cleavage-sensitive peptide and protein fragments in ionic-liquid-containing solvent systems. By applying the newly established [MMIM][Me2PO4]/buffer mixture as a reaction medium, significant advantages over purely aqueous or conventional organic solvent-containing media could be identified, including in particular the use of active wild-type proteases as biocatalysts, the suppression of any competitive proteolytic side reactions, the high turnover rates compared to classical organic solvents and the high stability of chemically labile reactants.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Peptídeo Hidrolases
/
Líquidos Iônicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article