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Freeze-drying as an alternative method of human sclera preservation.
Frota, Ana Carolina de Arantes; Lima Filho, Acácio Alves de Souza; Dias, Ana Beatriz Toledo; Lourenço, Andréia Cristina Dos Santos; Antecka, Emilia; Burnier, Miguel N.
Afiliação
  • Frota AC; Federal University of São Paulo, UNIFESP, São Paulo, Brazil. carolafrota@gmail. com
Arq Bras Oftalmol ; 71(2): 137-41, 2008.
Article em En | MEDLINE | ID: mdl-18516408
PURPOSE: To compare the effect of preserving sclera samples in either 95% ethanol or freeze-dried. METHODS: Ninety-six samples of human sclera were studied. Half of them were freeze-dried and half preserved in 95% ethanol. Preservation periods of 18, 45, 90 or 174 days were studied. Automated immunostaining was carried out in the Ventana BenchMarkR LT platform using collagen 1 and fibronectin antibodies. Histological staining was also performed with hematoxilin-eosin and Masson trichrome. Samples were classified according to the degree of collagen fiber parallelism (0-2), intensity of Masson staining (0-2), and the expression of both antibodies (0-3). Friedman and Wilcoxon tests were applied to compare preservation methods and p-values below 0.05 were considered to ensure statistical significance. RESULTS: Relevant results were found in three situations: (i) Friedman's test showed better collagen fiber integrity in the freeze-dried group rehydrated after 174-days as compared to the 90-day group; (ii) Wilcoxon's test showed better collagen fiber integrity in the freeze-dried group after 18 and 174 days as compared to the ethanol group; (iii) the freeze-dried group disclosed higher immunohistochemical expression for COL-1 antibody in the sclera samples rehydrated after 45, 90 and 174 days as compared to the ones rehydrated after 18 days. CONCLUSION: Histological and immunohistochemical analysis showed freeze-drying to be a superior method for sclera preservation as compared to 95% ethanol. This technique provides an easy method to manipulate tissue, with longer shelf life, and storage at room temperature.
Assuntos
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Base de dados: MEDLINE Assunto principal: Esclera / Etanol / Liofilização / Anti-Infecciosos Locais Tipo de estudo: Evaluation_studies / Observational_studies Limite: Humans Idioma: En Ano de publicação: 2008 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Esclera / Etanol / Liofilização / Anti-Infecciosos Locais Tipo de estudo: Evaluation_studies / Observational_studies Limite: Humans Idioma: En Ano de publicação: 2008 Tipo de documento: Article