[Y chromosome microdeletions of 664 Chinese men with azoospermia or severe oligozoospermia].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 25(3): 252-5, 2008 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-18543210
ABSTRACT
OBJECTIVE:
To explore the incidence and location of Y chromosome microdeletions in Chinese azoospermia and severe oligozoospermia, as well as the relationship between the deletion region and testicular phenotype.METHODS:
Semen samples or blood samples were collected from 664 Chinese patients (584 with azoospermia and 80 with severe oligozoospermia). DNA was extracted by incubating cells with a lysis buffer containing polymerase chain reaction (PCR) buffer and proteinase K, and was assayed for deletion of 15 sequence tagged sites (including 6 loci recommended by European Academy of Andrology and European Molecular Genetics Quality Network (EAA/EMQN) distributed in AZFa, AZFb and AZFc by 4 multiplex PCRs. The histological phenotypes of testes of some azoospermic patients harboring Y chromosome microdeletion were studied by fine needle aspiration.RESULTS:
Sixty-six (11.3%) cases of microdeletions were found in the 584 patients with azoospermia, and deletions of AZFc region are the leading group (72.7% of all deletions), followed by AZFbc (13.6%), AZFabc (6.1%), AZFb (4.5%) and AZFa (3.0%). In the 80 men with severe oligozoospermia, 10 (12.5%) cases of AZFc microdeletions were detected. While azoospermia (n=19) with AZFc region deletion showed variable testicular phenotype, deletions of AZFb+c and AZFa+b+c (n=7) resulted in severe impaired spermatogenesis characterized by Sertoli cell only syndrome and spermatogenic arrest at spermatogonia.CONCLUSION:
In the Chinese men with azoospermia and severe oligozoospermia, the incidence of Y chromosome microdeletions and the frequency of the deletions of the three AZF regions are similar to those described previously in other populations. Massive deletions of AZFb+c and AZFa+b+c impair spermatogenesis severely.
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Base de dados:
MEDLINE
Assunto principal:
Oligospermia
/
Deleção Cromossômica
/
Cromossomos Humanos Y
/
Azoospermia
Tipo de estudo:
Prognostic_studies
Limite:
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article