Biomarkers of neurotoxic shellfish poisoning.
Toxicon
; 52(2): 237-45, 2008 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-18582487
ABSTRACT
Urine specimens from patients diagnosed with neurotoxic shellfish poisoning (NSP) were examined for biomarkers of brevetoxin intoxication. Brevetoxins were concentrated from urine by using solid-phase extraction (SPE), and analyzed by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Urine extracts were fractionated by LC, and fractions analyzed for brevetoxins by ELISA. In subsequent LC-MS/MS analyses, several brevetoxin metabolites of B-type backbone were identified, with elution profiles consistent with those of ELISA. The more abundant brevetoxin metabolites in urine were characterized structurally by LC-MS/MS. With the exception of BTX-3, brevetoxin metabolites in urine differed from those found in shellfish and in shellfish meal remnants. Proposed structures of these major urinary metabolites are methylsulfoxy BTX-3, 27-epoxy BTX-3, and reduced BTX-B5. BTX-3 was found in all specimens examined. BTX-3 concentrations in urine, as determined by LC-MS/MS, correlated well with composite toxin measurements by ELISA (r(2)=0.96). BTX-3 is a useful biomarker for confirmation of clinical diagnosis of NSP.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Dinoflagellida
/
Bivalves
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Oxocinas
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Intoxicação por Frutos do Mar
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Doenças Transmitidas por Alimentos
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Toxinas Marinhas
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Neurotoxinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article