cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression.
Exp Cell Res
; 314(15): 2855-69, 2008 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-18644368
ABSTRACT
cAMP signaling is known to have significant effects on cell growth, either inhibitory or stimulatory depending on the cell type. Study of cAMP-induced growth inhibition in mammalian somatic cells has focused mainly on the combined role of protein kinase A (PKA) and mitogen-activated protein (MAP) kinases in regulation of progression through the G1 phase of the cell cycle. Here we show that cAMP signaling regulates histone H3 phosphorylation in a cell cycle-dependent fashion, increasing it in quiescent cells but dramatically reducing it in cycling cells. The latter is due to a rapid and dramatic loss of mitotic histone H3 phosphorylation caused by a disruption in G2 progression, as evidenced by the inhibition of mitotic entry and decreased activity of the CyclinB/Cdk1 kinase. The inhibition of G2 progression induced through cAMP signaling is dependent on expression of the catalytic subunit of PKA and is highly sensitive to intracellular cAMP concentration. The mechanism by which G2 progression is inhibited is independent of both DNA damage and MAP kinase signaling. Our results suggest that cAMP signaling activates a G2 checkpoint by a unique mechanism and provide new insight into normal cellular regulation of G2 progression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
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Fase G2
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AMP Cíclico
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Genes cdc
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Proteínas de Ciclo Celular
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Mitose
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article