A virus-based single-enzyme nanoreactor.
Nat Nanotechnol
; 2(10): 635-9, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-18654389
ABSTRACT
Most enzyme studies are carried out in bulk aqueous solution, at the so-called ensemble level, but more recently studies have appeared in which enzyme activity is measured at the level of a single molecule, revealing previously unseen properties. To this end, enzymes have been chemically or physically anchored to a surface, which is often disadvantageous because it may lead to denaturation. In a natural environment, enzymes are present in a confined reaction space, which inspired us to develop a generic method to carry out single-enzyme experiments in the restricted spatial environment of a virus capsid. We report here the incorporation of individual horseradish peroxidase enzymes in the inner cavity of a virus, and describe single-molecule studies on their enzymatic behaviour. These show that the virus capsid is permeable for substrate and product and that this permeability can be altered by changing pH.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Capsídeo
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Reatores Biológicos
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Nanotecnologia
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Biomimética
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Nanoestruturas
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Enzimas Imobilizadas
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Peroxidase do Rábano Silvestre
Idioma:
En
Ano de publicação:
2007
Tipo de documento:
Article