[The application of lentiviral vectors for tissue-specific gene manipulations].
Tsitologiia
; 50(4): 370-5, 2008.
Article
em Ru
| MEDLINE
| ID: mdl-18664120
ABSTRACT
The trophectoderm (TE) ofblastocysts, the first epithelium established in mammalian development, 1) plays signaling, supportive, and patterning functions during pre-implantation development, 2) ensures embryo implantation into the uterine wall, and 3) gives rise to extra-embryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive to lentiviral (LV) infection, represents a robust non-permeable physical barrier to the virus particles, thereby shielding the cells of the inner cell mass (ICM) from viral infection. This LV feature will allow modulations of gene expression in a lineage-specific manner, thus having significant applications in mouse functional genetics.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
/
Lentivirus
/
Vetores Genéticos
Limite:
Animals
/
Humans
Idioma:
Ru
Ano de publicação:
2008
Tipo de documento:
Article