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Synthesis and biological evaluation of a polysialic acid-based hydrogel as enzymatically degradable scaffold material for tissue engineering.
Berski, Silke; van Bergeijk, Jeroen; Schwarzer, David; Stark, Yvonne; Kasper, Cornelia; Scheper, Thomas; Grothe, Claudia; Gerardy-Schahn, Rita; Kirschning, Andreas; Dräger, Gerald.
Afiliação
  • Berski S; Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffe (BMWZ), Gottfried Willhelm Leibniz Universität Hannover, Schneiderberg 1B, 30167 Hannover, Germany.
Biomacromolecules ; 9(9): 2353-9, 2008 Sep.
Article em En | MEDLINE | ID: mdl-18690740
Restorative medicine has a constant need for improved scaffold materials. Degradable biopolymers often suffer from uncontrolled chemical or enzymatic hydrolysis by the host. The need for a second surgery on the other hand is a major drawback for nondegradable scaffold materials. In this paper we report the design and synthesis of a novel polysialic acid-based hydrogel with promising properties. Hydrogel synthesis was optimized and enzymatic degradation was studied using a phage-born endosialidase. After addition of endosialidase, hydrogels readily degraded depending on the amount of initially used cross-linker within 2 to 11 days. This polysialic acid hydrogel is not cytotoxic, completely stable under physiological conditions, and could be evaluated as growth support for PC12 cells. Here, additional coating with collagen I, poly-L-lysine or matrigel is mandatory to improve the properties of the material.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Siálicos / Hidrogéis / Engenharia Tecidual / Neuraminidase Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Siálicos / Hidrogéis / Engenharia Tecidual / Neuraminidase Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article