The development of a glove-box/Vitrobot combination: air-water interface events visualized by cryo-TEM.
Ultramicroscopy
; 108(11): 1478-83, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18691818
ABSTRACT
Aqueous interfaces are of paramount importance in the study of biological systems as well as in the biomedical sciences. To study these interfaces at the nanometer level it is of interest to develop methods that allow their observation with cryogenic transmission electron microscopy (cryo-TEM). Prevention of dehydration to preserve the "native" state during sample preparation prior to vitrification is often one of the most important parameters to control in cryo-TEM experiments. For the preparation of these types of samples, we felt the need for an extended workspace with temperature and humidity control; a 'glove-box' that seamlessly connects to the vitrification instrument, the Vitrobot. In this paper we describe the use of the glove-box in the 2D and 3D cryo-TEM study of DNA adsorption and calcium carbonate mineralization to Langmuir films. The data presented illustrates the necessity of a humidity-controlled environment to preserve the original "native" state of the monolayer system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tensoativos
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Carbonato de Cálcio
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DNA
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Microscopia Crioeletrônica
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article