A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.
Int J Biol Macromol
; 43(4): 352-8, 2008 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-18703082
ABSTRACT
The N-acetyl-galactosamine specific lectin from Macrotyloma axillare seeds (LMA) was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L(-1) fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pH-dependent assays showed best hemagglutinating activity at pH 6.0-8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sementes
/
Bioquímica
/
Lectinas de Plantas
/
Fabaceae
Tipo de estudo:
Health_economic_evaluation
Limite:
Humans
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article