Baculovirus surface display of E(rns) envelope glycoprotein of classical swine fever virus.
J Virol Methods
; 153(2): 149-55, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-18727937
ABSTRACT
Classical swine fever virus (CSFV) causes significant losses in the pig industry in many countries. E(rns) is an envelope glycoprotein of CSFV which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. In this study, one recombinant baculoviruses BacSC-E(rns) expressing histidine-tagged E(rns) with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evaluated in a mouse model. After infection, E(rns) was expressed and anchored on the plasma membrane of Sf-9 cells, as demonstrated by Western-blot and confocal microscopy. Immunogold electron microscopy demonstrated that the E(rns) glycoprotein was successfully displayed on the baculoviral envelope. Vaccine tests in animals showed that BacSC-E(rns) elicited significantly higher E(rns) antibody titers in the immunized mouse models than the control group. This demonstrates that the BacSC-E(rns) vaccine can be used potentially against CSFV infections. This is the first report demonstrating the potential of E(rns)-pseudotyped baculovirus as a CSFV vaccine.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
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Glicoproteínas de Membrana
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Vacinas Virais
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Proteínas do Envelope Viral
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Baculoviridae
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Peste Suína Clássica
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Vírus da Febre Suína Clássica
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article