A red-emitting naphthofluorescein-based fluorescent probe for selective detection of hydrogen peroxide in living cells.
Bioorg Med Chem Lett
; 18(22): 5948-50, 2008 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-18762422
ABSTRACT
We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600nm). Flow cytometry experiments in RAW264.7 macrophages establish that NPF1 can report changes in peroxide levels in living cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácidos Borônicos
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Transferência Ressonante de Energia de Fluorescência
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Corantes Fluorescentes
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Peróxido de Hidrogênio
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Macrófagos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article