Comparison of 3T3 fibroblasts growth on alginate and polyasparagine (PAA) scaffolds in mouse model.

ABSTRACT

INTRODUCTION: The aim of this study was to evaluate polyasparagine (PAA), a new, promising scaffold. PAA was compared with alginate, which is used in cell transplantations and may be regarded as a standard. MATERIALS AND METHODS: In vitro cell viability on both scaffolds was assessed. C57B1 mice were injected s. c. alginate or PAA with or without 3T3 cells. After two months specimens from sites of injection were examined and blood samples were taken for enzymatic activity estimation. Cathepsin D activity and alpha1-antitrypsin levels were measured. RESULTS: In vitro cell viability was lowest on PAA and highest in control group. Increase in levels of enzymes measured was observed in response to PAA and alginate and was lower in case of polymer seeded with cells. Increase in alpha1-antitrypsin levels was lower in case of PAA in comparison to alginate. Scaffold degradation in histopathological specimens was visible. CONCLUSION: The results indicate that PAA implants undergo biodegradation and nonspecific inflammatory response is comparable to alginate.